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Principle component analysis has been used to differentiate columns for use in chromatography for a number of years, *first developed by Euerby and Petersson based around tests suggested by Tanaka. A systematic approach to column charecterisation allows the analyst to choose from a diverse (or similar) range of columns.
*M.Euerby, P.Petersson, LC-GC Europe (Sept 2000) 665-677
High Selectivity – Retention factor ratio between Triphenylene and o-terphenyl Steric term based on shape selectivity. Useful for isomer and metabolite resolution
High Hydrophobicity – An indicator of ligand density & surface coverage. C18 is the most common ligand to retain a majority of compounds, however Evosphere C12 is highly selective as well, whilst Evosphere AQUA will retain both hydrophobic and hydrophilic molecules.
Ion-exchange shows when stationary phases have a charge. This can be used in conjunction with acid, basic functionalities to increase retention of polar analytes and provide orthoganol selectivity
Evosphere Physicals and Stationery Phases
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Fortis Technologies has eight reversed phase MFPP (Monodisperse Fully porous particles) stationary phases available:
| PRODUCT | PARTICLE SIZE | S.A | PORE SIZE | % CARBON | USP |
|---|---|---|---|---|---|
| Evosphere C18/PFP | 1.7um 3um & 5um | 350 m2/g | 100 A | 17 | L1 |
| Evosphere C18/AR | 1.7um 3um & 5um | 350 m2/g | 100 A | 17 | L1 |
| Evosphere RP-18 Amide | 1.7um 3um & 5um | 350 m2/g | 100 A | 20 | L60 |
| Evosphere Diphenyl | 1.7um 3um & 5um | 350 m2/g | 100 A | 15 | L11 |
| Evosphere Phenyl-Hexyl | 1.7um 3um & 5um | 350 m2/g | 100 A | 14 | L11 |
| Evosphere PFP | 1.7um 3um & 5um | 350 m2/g | 100 A | 13 | L43 |
| Evosphere AQUA | 1.7um 3um & 5um | 350 m2/g | 100 A | 18 | L96 |
| Evosphere C12 | 1.7um 3um & 5um | 350 m2/g | 100 A | 17 | L87 |
| Evosphere HILIC | 1.7um 3um & 5um | 350 m2/g | 100 A | n/a | L3 |
Evosphere AQUA
Evosphere C18/AR
Evosphere Diphenyl
Evosphere C18/PFP
Evosphere RP18-Amide
Evosphere PFP
Evosphere Phenyl-Hexyl
Evosphere C12
