Common HPLC Buffers

•••••

BufferpKaUsable pH Range
Trifluoroacetic acid
<<21.5 - 2.5
KH2PO4 / Phosphoric acid2.121.1 - 3.1
Tri-K-Citrate / hydrochloric acid 13.062.1 - 4.1
Potassium formate / formic acid3.82.8 - 4.8
Tri-K-Citrate / hydrochloric acid 24.73.7 - 5.7
Potassium acetate / acetic acid4.83.8 - 5.8
Tri-K-Citrate / hydrochloric acid 35.44.4 - 6.4
Ammonium formate3.8 & 9.22.8 - 4.8 & 8.2 - 10.2
Bis-tris propane HCl/Bis-tris propane6.85.8 - 7.8
Ammonium acetate4.8 & 9.23.8 - 5.8 & 8.2 - 10.2
KH2PO4 / K2HPO47.216.2 - 8.7
Tris HCl / Tris8.27.3 - 9.3
Bis-tris propane HCl/Bis-tris propane9.08.0 - 10.0
Ammonium hydroxide / Ammonia9.28.2 - 10.2
Borate (H3BO3 / Na2B4O7.10H2O)9.248.2 - 10.2
Glycine.HCl / glycine9.88.8 - 10.8
1-methylpiperidine.HCl / 1-methylpiperidine10.19.1 - 11.1
Diethylamine.HCl / diethylamine10.59.5 - 11.5
Triethylamine.HCl / triethylamine11.010.0 - 12.0
Pyrollidine.HCl/pyrollidine11.310.3 - 12.3

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