UHPLC – Backpressure

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Since 1.7um Fortis columns are the same surface area as their 3um and 5um counterparts, several advantages occur over lower surface area materials:

  • Loading of analyte concentration is not compromised
  • You can remove backpressure from the system by reducing column length, whilst still maintaining retention
  • You can increase resolution.

The choice is then open to use the high retention as a resolution benefit or to reduce the column length and therefore system backpressure :

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